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  2. Pearl V Ryder

Help! How does the Robust Background method work?

August 05, 2021

Pearl V. Ryder

 

The Robust Background algorithm is a powerful algorithm for automatically setting thresholds to segment objects of interest when your image contains mostly background. However...

Read More Help! How does the Robust Background method work?

Thinking like an image analyst, Part I: Project overview and data import

August 10, 2021

Pearl V. Ryder

One thing I’ve found very helpful in my journey as an image analyst is learning from others by observing their thought process when they tackle a new problem. In this series of five...

Read More Thinking like an image analyst, Part I: Project overview and data import

Thinking like an image analyst, Part II: Removing bright debris from analysis

August 17, 2021

Pearl V. Ryder

In the first post of this series, I gave an overview of this project and explained how I imported the data into CellProfiler. If you’d like to follow along in CellProfiler, the...

Read More Thinking like an image analyst, Part II: Removing bright debris from analysis

Thinking like an image analyst, Part III: Enhancing fibers for detection

September 01, 2021
 

Pearl V. Ryder

In the previous blog post in this series, I demonstrated how I masked out debris that was brighter than the fibers of interest. In this post, I’ll walk through how I enhanced the...

Read More Thinking like an image analyst, Part III: Enhancing fibers for detection

Thinking like an image analyst, Part IV: Detecting fibers as objects

November 05, 2021

Pearl V. Ryder

In the previous post in this “Thinking like an image analyst” series, I explained how I enhanced fibers to increase their brightness and applied a background subtraction to decrease...

Read More Thinking like an image analyst, Part IV: Detecting fibers as objects

Thinking like an image analyst, Part V: Making measurements and exporting the data for analysis

November 12, 2021

Pearl V. Ryder

In the previous posts in this series, I explained how I approached an image analysis project to segment extracellular fibrils, giving an overview of the project (Part I), showing how I...

Read More Thinking like an image analyst, Part V: Making measurements and exporting the data for analysis
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